Composite

Part:BBa_K5276032:Design

Designed by: Yuming Luo   Group: iGEM24_UCAS-China   (2024-10-01)


T7_Promoter-lacO-tetR-A118-lacI-Gp44


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2593
    Illegal EcoRI site found at 3703
    Illegal XbaI site found at 1492
    Illegal XbaI site found at 2159
    Illegal SpeI site found at 1127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2593
    Illegal EcoRI site found at 3703
    Illegal NheI site found at 3751
    Illegal SpeI site found at 1127
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2593
    Illegal EcoRI site found at 3703
    Illegal BglII site found at 2225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2593
    Illegal EcoRI site found at 3703
    Illegal XbaI site found at 1492
    Illegal XbaI site found at 2159
    Illegal SpeI site found at 1127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2593
    Illegal EcoRI site found at 3703
    Illegal XbaI site found at 1492
    Illegal XbaI site found at 2159
    Illegal SpeI site found at 1127
    Illegal NgoMIV site found at 2257
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 730